Bacteriostatic impact of nanoscale zero-valent iron against pathogenic bacteria in the municipal wastewater

Nanoscale zero-valent iron particles were investigated as an antibacterial agent against two Gram-positive bacteria; Staphylococcus aureus NRRL B-313 (S. aureus), Bacillus subtilus NRC (B. subtilus), and two Gram-negative bacteria; Escherichia coli NRC B-3703 (E. coli), Pseudomonas aeruginosa NRC B-32 (Ps. aeruginosa). The characterization of synthesized nZVI particles was obtained by XRD, SEM, EDX, and TG analyses. The results demonstrated that the nZVI particles have a spherical shape, mean crystalline size of 44.43 nm, and exhibited a good chemical and thermal stability performance under different physical conditions. The bacterial suspensions were subjected to the treatment using nZVI particle suspensions with a concentration of 10 mg/mL. The minimum inhibitory concentration of nZVI particles was determined using the well diffusion assay method and found to be 15, 10, 10, and 5 mg for the following four strains; S. aureus, B. subtilus, E. coli, and Ps. aeruginosa, respectively. The biological treatment results of municipal wastewater using nZVI particles revealed that the counts of total bacteria, total coliform, fecal coliform, S. aureus, fecal Streptococcus, and E. coli were decreased to 44.29%, 51.76%, 90.95%, 46.67%, 33.33%, and 93.89%, respectively, while the Ps. aeruginosa not detected in the wastewater sample. The enhanced inactivation performance of nZVI nanoparticles was mainly attributed to the reactive oxygen species (ROS) production, releasing of iron corrosion products like Fe²⁺/Fe³⁺ ions, and direct friction of nZVI particles with bacterial cells membranes. In addition, the nZVI particles presented a striking disinfection behavior in comparison with other widespread disinfection technologies such as chlorination. Accordingly, the obtained results introduce the nZVI particles as a promising disinfection technology.