[Amino acid analysis of proteins and physiological fluids in a single system of sodium-citrate buffer]

Programmes for automatic analysis of protein and peptide hydrolysates and physiological fluids in a single system of sodium citrate buffers are proposed. Retention time values of 59 ninhydrin-positive substances, including nonprotein amino acids, amino sugars (muramic acid, glucosamine, galactosamine, mannosamine and fucosamine) and corresponding aminopolyols, as well as three cysteine derivatives, viz. cysteic acid, S-carboxymethylcysteine and S-2-(pyridyl-4)ethylcysteine, have been determined. Several examples illustrate an increase of the resolution simply by change of the temperature programme of the column and (or) elution time.