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Distinct subpopulations of elicited human macrophages in peritoneal dialysis patients and women undergoing
H J Bos1, H van Bronswijk, T J Helmerhorst
1Department of Cell Biology, Medical Faculty, Free University, Amsterdam, The Netherlands.
Abstract:
The endogenous peroxidatic activity (PA) pattern of peritoneal macrophages from 24 continuous ambulatory peritoneal dialysis (CAPD) patients and from five healthy women undergoing laparoscopy was studied. In general, the macrophages showed two different PA patterns in vivo: exudate and negative macrophages. However, two of 24 CAPD patients showed resident macrophages, the first described in vivo in man. Since, in general, the examined human peritoneal macrophages are exudate and PA-negative, this suggests, in accordance with the animal model system, that a chronic sterile inflammation exists in the peritoneal cavity of CAPD patients and healthy women undergoing laparoscopy. After 2 hr culture, blood monocytes and peritoneal macrophages transformed into cells with the characteristics of exudate-resident and resident macrophages, so isolation procedures that include short periods of culture can change the developmental stage of human monocytes and macrophages.
Insights
Peritoneal macrophages in continuous ambulatory peritoneal dialysis (CAPD) patients and healthy individuals exhibit chronic sterile inflammation. Short culture periods can alter macrophage developmental stages, impacting research findings.
Area of Science:
- Immunology
- Cell Biology
- Nephrology
Background:
- Peritoneal macrophages play a crucial role in the peritoneal cavity's immune response.
- Understanding macrophage phenotypes in vivo is essential for interpreting their function.
- Continuous ambulatory peritoneal dialysis (CAPD) involves long-term interaction with the peritoneal membrane.
Purpose of the Study:
- To investigate the endogenous peroxidatic activity (PA) patterns of peritoneal macrophages in CAPD patients and healthy controls.
- To determine if CAPD is associated with chronic sterile inflammation in the peritoneal cavity.
- To assess the impact of short-term culture on human monocyte and macrophage phenotypes.
Main Methods:
- Analysis of peritoneal macrophages from 24 CAPD patients and 5 healthy women undergoing laparoscopy.
- Assessment of endogenous peroxidatic activity (PA) patterns in vivo.
- Observation of macrophage morphology and characteristics after 2 hours of in vitro culture.
Main Results:
- Peritoneal macrophages generally displayed exudate and PA-negative patterns in vivo.
- Two CAPD patients showed resident macrophages in vivo, a novel finding in humans.
- Short-term culture (2 hr) induced transformation of monocytes and macrophages towards exudate-resident and resident phenotypes.
Conclusions:
- The peritoneal environment in CAPD patients and healthy individuals likely harbors chronic sterile inflammation.
- The presence of resident macrophages in vivo in CAPD patients warrants further investigation.
- Short culture periods can artifactually alter the developmental stage of human monocytes and peritoneal macrophages, potentially confounding results.