CircRNA expression profiles and circRNA-miRNA-mRNA crosstalk in allergic rhinitis

Area of Science:

• Molecular Biology
• Immunology
• Genetics

Background:

• Circular RNAs (circRNAs) are implicated in inflammatory processes.
• The specific role of circRNAs in allergic rhinitis (AR) pathogenesis is not well understood.

Purpose of the Study:

• To analyze circRNA expression in AR.
• To identify a circRNA-miRNA-mRNA regulatory network in AR.
• To elucidate the molecular mechanisms underlying AR.

Main Methods:

• High-throughput sequencing (HTS) of circRNA, miRNA, and mRNA expression profiles in nasal mucosa.
• Bioinformatic and statistical analysis to construct a differentially expressed circRNA-miRNA-mRNA crosstalk network.
• RT-PCR and Sanger sequencing for circRNA validation.

Main Results:

• 264 differentially expressed circRNAs (DEcircRNAs) were identified in AR.
• A network comprising 11 DEcircRNAs, 17 miRNAs, and 29 mRNAs was constructed.
• Key pathways involved include Wnt signaling, TNF biosynthesis, and Toll-like receptors.

Conclusions:

• A novel DEcircRNA-DEmiRNA-DEmRNA network for AR was successfully constructed.
• This network provides a foundation for future research into AR molecular mechanisms.
• The identified network offers potential targets for AR therapeutic strategies.