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Prostaglandin E2 metabolism by human myometrium
R Cañete Soler1, A López Bernal, A C Turnbull
1University of Oxford, Nuffield Department of Obstetrics and Gynaecology, John Radcliffe Hospital, Headington, United Kingdom.
The Journal of Clinical Endocrinology and Metabolism
|February 1, 1988
Summary
Human myometrium metabolizes prostaglandin E2 (PGE2) into three main products, with PGEM being the most abundant. This metabolism, influenced by PG-9-oxo-reductase, may affect circulating prostaglandin levels during childbirth.
Area of Science:
- Reproductive biology
- Biochemistry
- Pharmacology
Background:
- Prostaglandins play a crucial role in reproductive processes, including parturition.
- Understanding prostaglandin metabolism in the myometrium is vital for comprehending uterine function.
Purpose of the Study:
- To investigate the metabolic pathways of prostaglandin E2 (PGE2) in human myometrium.
- To identify and quantify the metabolites of PGE2 produced by myometrial tissue.
Main Methods:
- Incubation of radiolabeled PGE2 with human myometrial homogenates.
- High-pressure liquid chromatography (HPLC) for metabolite separation.
- Radioimmunoassay (RIA) for precise quantification of metabolites.
Main Results:
- Three PGE2 metabolites were identified: PGF2 alpha, 13,14-dihydro-15-oxo-PGF2 alpha (PGFM), and 13,14-dihydro-15-oxo-PGE2 (PGEM).
- PGEM was the predominant metabolite, followed by PGF2 alpha and PGFM.
- Metabolite production was dependent on NADPH and inhibited by NADP.
- Metabolism patterns were consistent across pregnant and nonpregnant women and unaffected by labor status.
Conclusions:
- Human myometrium possesses PG-9-oxo-reductase activity.
- This enzymatic activity can influence circulating levels of PGEM and PGFM, especially during parturition.
- Myometrial prostaglandin metabolism is a significant factor in regulating local and systemic prostaglandin concentrations.