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Updated: Oct 30, 2025
Mass Spectrometry and Luminogenic-based Approaches to Characterize Phase I Metabolic Competency of In Vitro Cell Cultures
Published on: March 28, 2017
A Fluorometric CYP19A1 (Aromatase) Activity Assay in Live Cells
David K Heidary1, Sarah M Kriger1, Austin C Hachey1
1Department of Chemistry, University of Kentucky, 505 Rose St., Lexington, KY 40506, USA.
Abstract:
Inhibition of estrogen synthesis is an integral component of the frontline pharmacologic therapy for the treatment of estrogen receptor positive cancers. However, there is currently no direct, high-throughput-ready assay for aromatase (also known as CYP19A1) that can be performed in live cells. Herein we present a cell-based assay that allows for multiplexed assessment of enzyme activity, protein half-life, cell viability, and identification of inhibitors with slow off-rates.
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