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Updated: Oct 30, 2025
10:49
Assays for the Specific Growth Rate and Cell-binding Ability of Rotavirus
Published on: January 28, 2019
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Rotavirus research: 2014-2020.
Sarah Caddy1, Guido Papa2, Alexander Borodavka3
1Cambridge Institute for Therapeutic Immunology and Infectious Disease Jeffery Cheah Biomedical Centre, Cambridge, CB2 0AW, UK.
Virus Research
|July 5, 2021
Summary
Recent advances in rotavirus research, including reverse genetics and structural biology, offer new insights into viral replication and host interactions. This knowledge aids in developing strategies against rotavirus gastroenteritis in children and other species.
Area of Science:
- Virology
- Microbiology
- Immunology
Background:
- Rotaviruses are a leading cause of acute gastroenteritis in young children globally.
- They also affect various mammalian and avian species.
- Understanding rotavirus is crucial for public health and veterinary medicine.
Purpose of the Study:
- To review recent advancements in rotavirus research.
- To summarize structure-function studies of rotavirus proteins.
- To discuss viral replication, host interactions, diversity, immunity, and prevention.
Main Methods:
- Establishment of plasmid-only-based reverse genetics systems.
- Creation of human and mammalian intestinal enteroids.
- Application of cryo-electron microscopy (cryo-EM) and biophysical approaches.
Main Results:
- New insights into rotavirus structure-function relationships.
- Detailed understanding of viral replication mechanisms and host cell involvement.
- Characterization of viral genomic and antigenic diversity.
- Progress in understanding immune responses to rotavirus.
Conclusions:
- Recent technological advancements have significantly deepened our understanding of rotaviruses.
- This knowledge is vital for improving prevention and control strategies.
- Further research holds promise for combating rotavirus-associated diseases.
Keywords:
Antiviral compoundsCRISPRCsy4 genome editing in viroplasmsCellular compounds involved in rotavirus replicationCorrelates of protection against rotavirus diseaseFuture rotavirus researchFuture rotavirus vaccine candidatesMammalian intestinal enteroidsMolecular biology of Ca2+ release in rotavirusinfected cellsMolecular biology of replicationPlasmid only-based reverse geneticsRegulation of intercellular Ca2+ wavesRotavirus pre-genome assortment and packagingRotavirus vaccines - successes and challengesRotavirusesTranscription and replication by rotavirus RdRpViroplasms as protein-RNA condensates
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