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Characterization and Antioxidant Activity of Released Exopolysaccharide from Potential Probiotic Leuconostoc

Qing Zhang1, Jie Wang1, Qing Sun1

  • 1Key Laboratory of Food Biotechnology, College of Food and Bioengineering, Xihua University, Chengdu 610039, Sichuan, P.R. China.

Journal of Microbiology and Biotechnology
|July 6, 2021
PubMed

Insights

A novel probiotic strain, Leuconostoc mesenteroides subsp. mesenteroides LM187, was isolated from paocai. Its released exopolysaccharide (rEPS414) demonstrates significant cholesterol-lowering and antioxidant properties, indicating probiotic potential.

Area of Science:

  • Microbiology
  • Food Science
  • Biochemistry

Background:

  • Probiotics offer health benefits and are increasingly sought from fermented foods.
  • Exopolysaccharides (EPS) produced by bacteria can possess beneficial bioactivities.

Purpose of the Study:

  • To isolate and identify a novel probiotic strain from Sichuan paocai.
  • To characterize the released exopolysaccharide (rEPS) produced by the identified strain.
  • To evaluate the bioactivities of the rEPS for potential industrial applications.

Main Methods:

  • Isolation and identification of bacterial strain LM187 using microbiological and molecular techniques.
  • Purification and characterization of rEPS (rEPS414) including molecular weight, monosaccharide composition, and structural analysis (FTIR, NMR).
  • In vitro assessment of antioxidant activity and other functional properties like acid and bile salt resistance.

Main Results:

  • Strain LM187 identified as Leuconostoc mesenteroides subsp. mesenteroides, exhibiting acid and bile salt resistance.
  • Purified rEPS414 has a molecular weight of 7.757 × 10^5 Da, primarily composed of glucose with α- and β-glycosidic bonds.
  • rEPS414 displayed significant in vitro antioxidant activity and a porous, flake-like structure.

Conclusions:

  • Strain LM187 and its rEPS414 are promising candidates for probiotic applications.
  • The characterized rEPS414 possesses beneficial properties suitable for the food and health industries.
  • Further research into the in vivo efficacy and industrial application of rEPS414 is warranted.
Keywords:
CharacterizationLeuconostoc mesenteroidesantioxidantreleased exopolysaccharide

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