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Updated: Oct 29, 2025

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A fluorescent probe for STED microscopy to study NIP-specific B cells

Selda Kabatas Glowacki1, Maria Angela Gomes de Castro, Ka Man Yip

  • 1Institute of Neuro- and Sensory Physiology, University Medical Center Göttingen, 37073, Göttingen, Germany.

The Analyst
|July 6, 2021
PubMed

We have developed a series of monovalent fluorophore-conjugated affinity probes based on the hapten 3-nitro-4-hydroxy-5-iodophenylacetyl (NIP), which is widely used as a model antigen to study B lymphocytes and the functional principles of B cell antigen receptors (BCRs). We successfully used them in flow-cytometry, confocal and super-resolution microscopy techniques.

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