[Functional discovery and production technology for natural bioactive peptides]

Area of Science:

• Biochemistry
• Peptide Science
• Food Science

Background:

• Bioactive peptides offer health benefits like reduced blood pressure, blood sugar, and lipids, plus anti-obesity and anti-cancer effects.
• Efficient methods for discovering and producing these peptides are crucial for their application in health and functional foods.

Purpose of the Study:

• To review the advancements in function discovery and production technologies for natural bioactive peptides.
• To compare the top-down and bottom-up approaches for peptide discovery and production.
• To propose a combined strategy for improved natural bioactive peptide development.

Main Methods:

• Review of existing literature on top-down (direct extraction, hydrolysis, fermentation) and bottom-up (modification, database mining, synthesis, recombinant expression) approaches.
• Analysis of the advantages and limitations of each approach for functional food and drug development.
• Integration of proteomic information with sequencing and mass spectrometry for a hybrid approach.

Main Results:

• Top-down methods involve direct extraction and identification, facing challenges like complexity, low efficiency, high cost, and quality control issues.
• Bottom-up methods include polypeptide modification and database mining, suitable for peptide drugs but less so for functional foods.
• Combining top-down and bottom-up approaches, aided by advanced sequencing and mass spectrometry, can overcome individual limitations.

Conclusions:

• A combined top-down and bottom-up strategy, leveraging proteomic data, presents a novel approach for the rapid development and production of natural bioactive peptides.
• This integrated strategy addresses the limitations of traditional methods, paving the way for enhanced applications in health and nutrition.
• Further research into optimizing this combined approach is essential for realizing the full potential of bioactive peptides.