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An unusual case of anti-D detection in two consecutive D+ patient samples: Antibody carryover on an automated gel
Kimberly Gabert1, Jansen N Seheult2,3, Michael P Meyer4
1Department of Reference and Transfusion Services, IRL, Vitalant, Pittsburgh, Pennsylvania, USA.
Background:
Laboratory results can be affected by sample to sample carryover. Carryover of different analytes occurring in automated clinical chemistry, immunology, hematology, and molecular laboratories is well described. However, carryover in a transfusion service laboratory is not reported in medical literature.
Materials And Methods:
Immunohematology testing results, demographic data, and clinical data were reviewed on three patients retrospectively from 2015 to 2019.
Results:
Type and screen samples tested on automated gel platform from two D+ patients were affected by anti-D carryover from a patient sample with a very high-titer anti-D. Additional immunohematology and molecular testing confirmed that anti-D in samples of two D+ patients was due to carryover.
Conclusion:
A case of anti-D carryover caused false detection of anti-D in two D+ patients. Carryover can have implications for patient management. Transfusion laboratory staff need to be aware of it and investigate any unexpected results further.
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