Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

UV–Vis Spectroscopy: Molecular Electronic Transitions01:16

UV–Vis Spectroscopy: Molecular Electronic Transitions

2.1K
In Ultraviolet–Visible (UV–Vis) spectroscopy, the absorption of electromagnetic radiation is used to probe the electronic structure of molecules. This technique provides insights into molecular electronic transitions, particularly the movement of electrons between different molecular orbitals. Radiation is absorbed if the energy of the electromagnetic radiation passing through the molecule is precisely equal to the energy difference between the excited and ground states. During this...
2.1K
Atomic Spectroscopy: Effects of Temperature01:27

Atomic Spectroscopy: Effects of Temperature

577
Atomization, converting samples into gas-phase atoms and ions, is essential for atomic spectroscopy. The flame temperature required for atomization affects the efficiency of the atomic spectroscopic methods by increasing the atomization efficiency and the relative population of the excited and ground states.
At thermal equilibrium, the relative populations of excited and ground state atoms can be estimated using the Maxwell–Boltzmann distribution. For example, an increase in temperature...
577
¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR01:15

¹H NMR of Conformationally Flexible Molecules: Variable-Temperature NMR

1.3K
The axial and equatorial protons in cyclohexane can be distinguished by performing a variable-temperature NMR experiment. In this process, except for one proton, the remaining eleven protons are replaced by deuterium. The deuterium substitution avoids the possible peak splitting caused by the spin-spin coupling between the adjacent protons. The remaining proton flips between the axial and equatorial positions.
1.3K
IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration01:16

IR Spectroscopy: Hooke's Law Approximation of Molecular Vibration

2.1K
A covalently bonded heteronuclear diatomic molecule can be modeled as two vibrating masses connected by a spring. The vibrational frequency of the bond can be expressed using an equation derived from Hooke's law, which describes how the force applied to stretch or compress a spring is proportional to the displacement of the spring. In this case, the atoms behave like masses, and the bond acts like a spring.
According to Hooke's law, the vibrational frequency is directly proportional to...
2.1K
UV–Vis Spectroscopy of Conjugated Systems01:32

UV–Vis Spectroscopy of Conjugated Systems

7.7K
Organic compounds with conjugated double bonds show strong absorption features in the UV–visible region of the electromagnetic spectrum attributed to π → π* electronic excitations. Generally, a UV–vis absorption spectrum is recorded as a plot of absorbance vs wavelength. The wavelength of maximum absorbance, which manifests as a peak in the absorption spectrum, is denoted as λmax.
One of the factors influencing λmax is the extent...
7.7K
Molecular Spectroscopy: Absorption and Emission01:14

Molecular Spectroscopy: Absorption and Emission

3.8K
Molecules possess discrete energy levels called quantum states. Unlike atoms, which have simpler energy levels, molecules possess additional rotational and vibrational energy levels.  Each energy level is separated by an energy gap, with the gaps between adjacent electronic, vibrational, and rotational levels varying significantly. The three types of energy levels in a diatomic molecule are shown in Figure 1.
3.8K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Letter: A Histology-Defined Triple-Negative Phenotype of Primary Biliary Cholangitis.

Alimentary pharmacology & therapeutics·2026
Same author

Exploring the third dimension in quantum confinement of surface electrons.

Science advances·2026
Same author

Red OLED with efficiency of 25.6% at 10,000 cd m<sup>-2</sup> based on selenium embedding multiple resonance framework.

Light, science & applications·2026
Same author

Guiding the Formation of Surface-Confined 2D Metal-Organic Coordination Networks by Variation of Constituent Landing Energy in Electrospray Deposition of an Iron(II) Grid Complex.

Small methods·2026
Same author

Vibrational-State-Regulated Hot-Band Thermal Activation for Anti-Stokes Luminescence.

The journal of physical chemistry letters·2026
Same author

Landing-Energy-Controlled Surface Conformation of Electrosprayed Foldamer Molecules on Au(111).

ACS nano·2026
Same journal

Biodegradable Self-Powered Electrotherapy Patch for Integrated Smart Wound Management.

Analytical chemistry·2026
Same journal

Metabolite Fraction Libraries for Quantitative NMR Metabolomics.

Analytical chemistry·2026
Same journal

Self-Contained Lateral-Flow Microfluidic Bead-Based Assay for Rapid Quantification of Early-Stage Kidney Biomarkers.

Analytical chemistry·2026
Same journal

Overcoming the Debye Shielding Effect with Concave-Convex Structures for Sensitivity-Enhanced Thin-Film Transistors.

Analytical chemistry·2026
Same journal

Mode-Phase-Difference Photothermal Spectroscopy Assisted by a Bent Biconically Tapered Microfiber for Gas Sensing.

Analytical chemistry·2026
Same journal

Negative-Pressure-Actuated Microfluidics: A Dual-Mode Point-of-Care Sensor for Allergen-Specific IgE in Interstitial Fluid.

Analytical chemistry·2026
See all related articles

Related Experiment Video

Updated: Oct 29, 2025

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.9K

Single Molecules in Strong Optical Fields: A Variable-Temperature Molecular Junction Spectroscopy Setup.

Hai Bi1, Chao Jing1,2,3, Peter Hasch1

  • 1Physics-Department E20, Technical University of Munich, James Franck Str. 1, 85748 Garching, Germany.

Analytical Chemistry
|July 7, 2021
PubMed
Summary
This summary is machine-generated.

Researchers developed a new optical near-field technique to study single-molecule junctions. This method simultaneously analyzes electronic and optical properties, advancing molecular electronics development.

More Related Videos

Single-Molecule F&#246;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

9.7K
Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

9.5K

Related Experiment Videos

Last Updated: Oct 29, 2025

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures
08:53

Angle-resolved Photoemission Spectroscopy At Ultra-low Temperatures

Published on: October 9, 2012

17.9K
Single-Molecule F&#246;rster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1
11:27

Single-Molecule Förster Resonance Energy Transfer Methods for Real-Time Investigation of the Holliday Junction Resolution by GEN1

Published on: September 18, 2019

9.7K
Spatial Separation of Molecular Conformers and Clusters
10:37

Spatial Separation of Molecular Conformers and Clusters

Published on: January 9, 2014

9.5K

Area of Science:

  • Molecular electronics
  • Nanotechnology
  • Spectroscopy

Background:

  • Understanding electron transport in single molecules is crucial for molecular electronic devices.
  • Current methods like I-V characteristics provide limited information on molecular junctions.
  • Multiparameter studies are needed to analyze chemical bonds, geometry, and strain.

Purpose of the Study:

  • To develop an advanced analytical method for investigating single-molecule junctions.
  • To enable simultaneous analysis of electronic and optical properties at variable temperatures.
  • To explore previously uncharacterized properties like photoconductance and photocurrent generation.

Main Methods:

  • Development of an optical near-field technique using an apertureless near-field emitter.
  • Utilizing the emitter as a counter electrode and plasmonic waveguide.
  • Focusing surface plasmon polaritons to create a confined evanescent field around the molecular junction.

Main Results:

  • Demonstrated proof of concept for the multichannel method at low temperatures.
  • Simultaneously investigated electronic and optical features of p-terphenyl-4,4″-dithiol.
  • Showcased the method's ability to analyze molecule properties based on charge state.

Conclusions:

  • The developed multichannel method enhances the understanding of single-molecule junctions.
  • This technique allows for spectroscopic characterization of molecular junctions.
  • It opens avenues for studying photoconductance and photocurrent generation in molecular systems.