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Updated: Oct 29, 2025

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies
05:30

Author Spotlight: Advancing the Analysis of Plasma Extracellular Vesicle Proteome for Cardiovascular Biomarker Studies

Published on: January 31, 2025

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Extracellular vesicles in cardiovascular disease

Chenyuan Huang1, Yub Raj Neupane2, Xiong Chang Lim3

  • 1Department of Surgery, Yong Loo Lin School of Medicine, National University of Singapore, Singapore, Singapore; Cardiovascular Research Institute, National University Heart Centre Singapore, Singapore, Singapore.

Advances in Clinical Chemistry
|July 7, 2021
PubMed

Insights

Extracellular vesicles (EVs) show promise for cardiovascular disease diagnostics and therapeutics. Challenges in EV isolation and standardization hinder their clinical application, but ongoing research offers potential solutions.

Area of Science:

  • Biomedical Science
  • Nanotechnology
  • Cardiovascular Research

Background:

  • Cardiovascular disease is a leading global health concern.
  • Extracellular vesicles (EVs) are emerging as valuable tools for liquid biopsy in cardiovascular disease.
  • EVs offer potential as cell-free therapeutics and drug delivery systems due to their bioactive cargo and intercellular transfer capabilities.

Purpose of the Study:

  • To review recent advances in EV-based biomarker discovery for cardiovascular disease.
  • To highlight the therapeutic and drug delivery potential of EVs and EV mimetics in cardiovascular disease.
  • To discuss current challenges and potential alternatives in EV isolation, purification, and fabrication.

Main Methods:

  • Literature review of recent advancements in extracellular vesicle research.
  • Analysis of current methodologies for EV isolation, purification, and characterization.
  • Exploration of therapeutic and drug delivery applications of EVs in cardiovascular disease.

Main Results:

  • EVs show significant potential for biomarker discovery in cardiovascular disease.
  • EVs and EV mimetics hold promise for cell-free therapeutics and drug delivery systems.
  • Significant challenges remain in EV standardization, isolation, and production yield.

Conclusions:

  • EVs represent a promising frontier for cardiovascular disease diagnostics and therapeutics.
  • Standardization of EV isolation and purification methods is crucial for clinical translation.
  • Further research into alternative fabrication and production methods is needed to overcome current limitations.

Keywords:
AtherosclerosisCardiovascular diseaseDiagnosisDrug delivery systemExosomeExtracellular vesicleMicrovesicleMyocardial infarctionStrokeTheranosticsTherapeutics

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