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Related Concept Videos

Sanger Sequencing01:57

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DNA sequencing is a fundamental technique that is routinely used in the biological sciences. This method can be applied to a range of questions at different scales - from the sequencing of a cloned DNA fragment or the study of a mutation in a gene up to whole-genome sequencing. However, despite the widespread use of sequencing today, it was not until 1977 that Fredrick Sanger and his collaborators developed the chain-termination method to decode DNA sequences. It relies on the separation of a...
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RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
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Related Experiment Video

Updated: Oct 29, 2025

Efficient SARS-CoV-2 Quantitative Reverse Transcriptase PCR Saliva Diagnostic Strategy utilizing Open-Source Pipetting Robots
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Sequencing SARS-CoV-2 Genomes from Saliva.

Tara Alpert1, Chantal B F Vogels1, Mallery I Breban1

  • 1Department of Epidemiology of Microbial Diseases, Yale School of Public Health, New Haven, CT 06510, USA.

Medrxiv : the Preprint Server for Health Sciences
|July 7, 2021
PubMed
Summary

Genomic sequencing of SARS-CoV-2 from saliva is feasible and yields results comparable to nasopharyngeal swabs. RNA extraction is essential for obtaining complete viral genomes from saliva samples.

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Area of Science:

  • Virology
  • Genomics
  • Epidemiology

Background:

  • Genomic sequencing is vital for tracking SARS-CoV-2 evolution and spread.
  • Nasopharyngeal swabs are the standard specimen for viral sequencing.
  • Saliva is a viable alternative for SARS-CoV-2 detection but less common for sequencing.

Conclusions:

  • Saliva is a suitable and effective specimen for SARS-CoV-2 genomic sequencing.
  • The ARTIC protocol and Nanopore sequencing are effective with saliva.
  • RNA extraction is critical for maximizing genomic data recovery from saliva specimens.