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Updated: Oct 29, 2025

Large-Scale SARS-CoV-2 Testing Utilizing Saliva and Transposition Sample Pooling
Published on: June 23, 2022
SARS-CoV-2 nucleic acid shedding is variable for every person
Daiana de Lima-Morales1,2, Priscila Lamb Wink1,2,3, Fabiana Caroline Zempulski Volpato1,2,4
1Hospital de Clínicas de Porto Alegre, Centro de Pesquisa Experimental, Laboratório de Pesquisa em Resistência Bacteriana (Labresis), Porto Alegre, RS, Brasil.
Introduction:
Considering the persistent positivity on RT-qPCR tests, the results of SARS-CoV-2 were monitored to evaluate the viral RNA shedding period.
Methods:
Between March and June 2020, the sequential results of 29 healthcare workers' were monitored using RT-qPCR.
Results:
More than 50% of the individuals remained RT-qPCR positive after 14 days. Furthermore, this is the first study to describe positive RT-qPCR for SARS-CoV-2 in a healthcare worker with mild symptoms 95 days after the first positive test.
Conclusions:
Sequential RT-qPCR results were heterogeneous, and the viral RNA shedding period is unique for each person.
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