Paraliobacillus salinarum sp. nov., isolated from saline soil in Yingkou, China

A novel Gram-stain-positive, facultatively aerobic, slightly halophilic, endospore-forming bacterium, designated G6-18^T, was isolated from saline soil collected in Yingkou, Liaoning, PR China. Cells of strain G6-18^T grew at 10-37 °C (optimum, 30 °C), at pH 6.0-9.0 (optimum, pH 8.0) and in the presence of 2-15 % (w/v) NaCl (optimum, 5 %). The strain could be clearly distinguished from the related species of the genus Paraliobacillus by its phylogenetic position and biochemical characteristics. It presented MK-7 as the major quinone and the dominant cellular fatty acids were iso-C_16 : 0, anteiso-C_15 : 0, C_16 : 0 and iso-C_14 : 0. The polar lipids consisted of diphosphatidylglycerol and phosphatidylglycerol as the major components. The G+C content of strain G6-18^T genome was 35.3 mol%. 16S rRNA analysis showed that strain G6-18^T had the highest similarity to Paraliobacillus ryukyuensis DSM 15140^T, reaching 97.0 %, followed by Paraliobacillus quinghaiensis CGMCC 1.6333^T with a value of 96.3 %. The average nucleotide identity values between strain G6-18^T and Paraliobacillus ryukyuensis DSM 15140^T, Paraliobacillus sedimins KCTC 33762^T, Paraliobacillus quinghaiensis CGMCC 1.6333^T and Paraliobacillus zengyii DSM 107811^T were 74.3, 72.0, 73.2 and 72.8 %, respectively, and the digital DNA-DNA hybridization values between strain G6-18^T and the neighbouring strains were 15.6, 13.8, 14.2 and 14.2 %, respectively. Based on phenotypic, chemotaxonomic and phylogenetic inferences, strain G6-18^T represents a novel species of the genus Paraliobacillus, for which the name Paraliobacillus salinarum sp. nov. (=CGMCC 1.12058^T=DSM 25428^T) is proposed.