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Microstructured titanium functionalized by naringin inserted multilayers for promoting osteogenesis and inhibiting

Ke Shen1, Xiaojing Zhang1, Qiang Tang1

  • 1School of Life Science, Jiangsu Normal University, Xuzhou, Jiangsu, China.

Journal of Biomaterials Science. Polymer Edition
|July 7, 2021
PubMed
Summary

This study developed a naringin-coated microstructured titanium implant to improve fracture repair in osteoporosis. The implant enhanced bone formation and reduced bone resorption, offering a novel solution for patients.

Keywords:
Titaniumlayer by layernaringinosteoclastogenesisosteogenesis

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Area of Science:

  • Biomaterials Science
  • Orthopedic Surgery
  • Regenerative Medicine

Background:

  • Osteoporosis significantly increases fracture risk, complicating repair due to reduced bone volume.
  • Titanium implants are often necessary for fracture fixation in osteoporotic patients.
  • Current treatments lack sufficient osteogenic and anti-resorptive properties for effective bone healing.

Purpose of the Study:

  • To design and evaluate a novel slow-release system using microstructured titanium (Micro-Ti) functionalized with naringin (NA) multilayers.
  • To investigate the effects of this modified titanium surface on osteoblast differentiation and osteoclastogenesis in vitro.
  • To assess the potential of this approach for enhancing fracture repair in osteoporosis.

Main Methods:

  • Microstructured titanium (Micro-Ti) surfaces were created using dual acid etching.
  • Naringin (NA), chitosan (CHI), and gelatin (GEL) multilayers were applied via layer-by-layer assembly, forming LBL (NA) coated-Ti.
  • Osteoblasts (ME3T3-E1) and macrophages (RAW 264.7) were cultured on untreated and treated titanium surfaces for in vitro analysis.

Main Results:

  • LBL (NA) coated-Ti surfaces promoted higher osteoblast alkaline phosphatase (ALP) activity and mineralization.
  • Gene expression analysis showed increased osteogenic markers (Runx2, ALP, Col I, OCN, OPN, OPG) on LBL (NA) coated-Ti.
  • Significant reduction in osteoclast activity and differentiation markers (CTSK, NFAT, TRAP, VATP) was observed on LBL (NA) coated-Ti surfaces.

Conclusions:

  • Microstructured titanium functionalized with naringin multilayers effectively enhances osteoblast differentiation.
  • This modified titanium surface significantly inhibits osteoclast formation and activity.
  • The developed approach offers a promising strategy for improving titanium-based implants for fracture repair in osteoporosis patients.