Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sociodemographic Factors, but Not Rurality, Explain Limited Variance in Fibromyalgia Severity: A Secondary Analysis of Two Randomized Clinical Trials.

Pain medicine (Malden, Mass.)·2026
Same author

Urobiome Analysis in Interstitial Cystitis/Bladder Pain Syndrome Reveals Nuanced Differences Associated with Localized Pain.

International urogynecology journal·2026
Same author

BCL6 in T cells promotes type 1 diabetes by redirecting fates of insulin-autoreactive B lymphocytes.

iScience·2026
Same author

Idiopathic Inflammatory Myopathy Related Interstitial Lung Disease Patients Present to Pulmonology: A Tertiary Center Experience.

Annals of the American Thoracic Society·2026
Same author

B cells in anti-tRNA synthetase syndrome patients show an activated, interferon-responsive signature.

Frontiers in immunology·2026
Same author

Prefusion-specific glycoprotein B human antibodies protect against neonatal HSV-2 infection.

bioRxiv : the preprint server for biology·2026

Related Experiment Video

Updated: Oct 29, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

12.5K

High-Throughput Detection of Autoantigen-Specific B Cells Among Distinct Functional Subsets in Autoimmune Donors.

Bryan A Joosse1, James H Jackson1,2, Alberto Cisneros1

  • 1Department of Medicine, Division of Rheumatology and Immunology, Vanderbilt University Medical Center, Nashville, TN, United States.

Frontiers in Immunology
|July 8, 2021
PubMed
Summary

Antigen-specific B cells (ASBCs) drive autoimmune diseases. A new pipeline identifies and expands ASBCs from patient samples, revealing their role in type 1 diabetes and anti-histidyl tRNA synthetase syndrome.

Keywords:
B cell receptor (BCR)B cellsSjogren’s syndromeautoantigenautoimmune diseasemyositissystemic sclerosis (scleroderma)type 1 diabetes

More Related Videos

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

13.0K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

2.8K

Related Experiment Videos

Last Updated: Oct 29, 2025

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function
09:25

Detection and Enrichment of Rare Antigen-specific B Cells for Analysis of Phenotype and Function

Published on: February 16, 2017

12.5K
Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies
10:16

Generation of Two-color Antigen Microarrays for the Simultaneous Detection of IgG and IgM Autoantibodies

Published on: September 15, 2016

13.0K
A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases
06:50

A High-Throughput Electrochemiluminescence 7-Plex Assay Simultaneously Screening for Type 1 Diabetes and Multiple Autoimmune Diseases

Published on: May 29, 2020

2.8K

Area of Science:

  • Immunology
  • Autoimmunity
  • B cell biology

Background:

  • Antigen-specific B cells (ASBCs) play a critical role in autoimmune diseases by presenting autoantigens and differentiating into autoantibody-producing cells.
  • Autoantibodies are diagnostic markers, but ASBCs can drive disease independently of autoantibody secretion, highlighting a disconnect.
  • Studying ASBCs directly is crucial for developing effective therapies for autoimmune conditions.

Purpose of the Study:

  • To develop a high-throughput screening pipeline for identifying, expanding, and characterizing ASBCs from patient samples.
  • To investigate the capacity of various B cell subsets to differentiate into antibody-secreting cells.
  • To analyze the phenotypic and repertoire attributes of ASBCs in autoimmune diseases like type 1 diabetes and anti-histidyl tRNA synthetase syndrome.

Main Methods:

  • A high-throughput screening pipeline was established to phenotypically identify, expand *in vitro*, and stimulate B cell subsets.
  • Enzyme-linked immunosorbent assay (ELISA) was used to detect antibody secretion and identify wells enriched for ASBCs.
  • The pipeline was applied to samples from patients with type 1 diabetes and anti-histidyl tRNA synthetase syndrome.

Main Results:

  • The pipeline successfully expanded several B cell subsets, including IgM+, IgD+, CD27- memory, and switched memory B cells, into antibody-secreting cells.
  • Bimodal responses were observed in CD21lo B cell subsets, indicating heterogeneity within this population.
  • Insulin-binding B cell bias was detected in CD27- and CD27+ memory subsets in pre-symptomatic type 1 diabetes donors.
  • Jo-1-binding B cells and total B cells from anti-histidyl tRNA synthetase syndrome patients expanded significantly (20-30 fold).

Conclusions:

  • The developed technology enables the interrogation of phenotypic and repertoire attributes of ASBCs from small numbers of cryopreserved peripheral blood mononuclear cells.
  • This approach is valuable for studying ASBCs in autoimmune diseases, offering insights beyond autoantibody detection.
  • The findings highlight the potential of ASBCs as therapeutic targets for autoimmune conditions.