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Related Experiment Video

Updated: Oct 29, 2025

Isolating, Sequencing and Analyzing Extracellular MicroRNAs from Human Mesenchymal Stem Cells
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Distinct Extracellular RNA Profiles in Different Plasma Components.

Jing Jia1,2, Shangdong Yang1, Jinyong Huang2

  • 1Department of Medical Genetics & Cell Biology, School of Basic Medical Sciences, Zhengzhou University, Zhengzhou, China.

Frontiers in Genetics
|July 8, 2021
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Plasma fractions contain distinct extracellular RNA (exRNA) profiles. Understanding these differences is crucial for developing reliable exRNA biomarkers for diagnostics and prognostics.

Keywords:
RNA-seqexRNAligation-freeliquid biopsyplasma

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Area of Science:

  • Molecular Biology
  • Biochemistry
  • Genomics

Background:

  • Circulating extracellular RNAs (exRNAs) show promise as biomarkers.
  • Limited knowledge exists regarding exRNA differences across various plasma fractions.

Purpose of the Study:

  • To characterize and compare RNA species distribution in distinct plasma fractions.
  • To investigate the potential of plasma fractions as sources for exRNA biomarker discovery.

Main Methods:

  • Collected four plasma fractions (platelets/debris, thrombin precipitates, extracellular vesicles, supernatant) from 10 samples.
  • Prepared ligation-free libraries for full-spectrum RNA profiling without size selection or rRNA reduction.
  • Employed a stepwise alignment strategy to categorize diverse RNA species (miRNAs, lncRNAs, circRNAs, etc.).

Main Results:

  • Each plasma fraction exhibited a unique RNA species distribution.
  • Hierarchical clustering revealed distinct profiles between fractions and similarities within the same fraction.
  • Novel transcripts and predicted microRNAs (miRNAs) were identified in specific plasma fractions.

Conclusions:

  • RNA species distribution and functional RNA content are dependent on the plasma fraction.
  • Careful plasma preparation and analysis of different fractions are essential for robust exRNA biomarker studies.