Related Experiment Video

Updated: Oct 29, 2025

The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

112.6K

PSMD7 downregulation suppresses lung cancer progression by regulating the p53 pathway

Xinchun Xu1, Xiaofeng Xuan2, Jieru Zhang2

  • 1Department of Ultrasound, The Affiliated Zhangjiagang Hospital of Soochow University, 68 Jiyang West Road, Suzhou, 215600, China.

Journal of Cancer
|July 8, 2021
PubMed

Insights

The deubiquitinase PSMD7 is highly expressed in non-small cell lung cancer (NSCLC), promoting tumor growth and progression. Inhibiting PSMD7 offers a potential therapeutic strategy for lung adenocarcinoma (LUAD).

Area of Science:

  • Oncology
  • Molecular Biology
  • Biochemistry

Background:

  • Lung cancer is a leading cause of cancer-related deaths globally.
  • The deubiquitinase PSMD7 is a key component of the 26S proteasome, involved in protein degradation.
  • The role of PSMD7 in non-small cell lung cancer (NSCLC) progression is not well understood.

Purpose of the Study:

  • To investigate the expression and function of PSMD7 in non-small cell lung cancer (NSCLC).
  • To determine if PSMD7 can serve as a prognostic biomarker and therapeutic target in lung adenocarcinoma (LUAD).

Main Methods:

  • PSMD7 expression analysis in NSCLC tissues and adjacent noncancerous tissues.
  • PSMD7 knockdown experiments in LUAD cell lines and xenograft mouse models.
  • Cell cycle, apoptosis, and senescence assays.
  • Western blot analysis to assess protein expression levels.

Main Results:

  • PSMD7 expression is significantly upregulated in NSCLC tissues compared to normal tissues.
  • High PSMD7 expression correlates with lymph node metastasis and poorer overall survival (OS) and disease-free survival (DFS) in LUAD patients.
  • PSMD7 knockdown inhibits LUAD cell proliferation, induces cell cycle arrest, senescence, and apoptosis.
  • PSMD7 depletion increases p53 levels and downstream targets (p21, PUMA) in a p53-dependent manner.
  • PSMD7 knockdown significantly suppresses LUAD tumor growth in vivo.

Conclusions:

  • PSMD7 is oncogenic in NSCLC, promoting tumor progression and inhibiting apoptosis.
  • PSMD7 is a potential prognostic biomarker for LUAD.
  • Targeting PSMD7 represents a promising therapeutic strategy for LUAD.
Keywords:
LUADNSCLCPSMD7cell cycle proteinsdeubiquitinasep53

More Related Videos

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma10:21

Author Spotlight: Exploring the Role of Inflammation in the Co-occurrence of Primary Sjogren's Syndrome and Lung Adenocarcinoma

Published on: September 20, 2024

583
Yeast As a Chassis for Developing Functional Assays to Study Human P5314:57

Yeast As a Chassis for Developing Functional Assays to Study Human P53

Published on: August 4, 2019

9.7K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
4.8K
Interactions Between Signaling Pathways01:19

Interactions Between Signaling Pathways

Signaling cascades usually lack linearity. Multiple pathways interact and regulate one another, allowing cells to integrate and respond to diverse environmental stimuli.
Convergence and divergence, and cross-talk between signaling pathways
Two distinct signaling pathways can converge on a single functional unit, which may either be a single protein or a complex of proteins. The response is either functionally distinct or synergistic between the two pathways but different from the response...
6.7K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
36.7K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
4.0K
Receptor Downregulation in MVBs01:15

Receptor Downregulation in MVBs

Multivesicular bodies (MVBs) are mature endosomes that sort ubiquitinated proteins and then fuse with lysosomes to degrade the sorted proteins. Epidermal growth factor (EGF) and its receptor (EGFR) form a complex that can be internalized through endocytosis, sorted into an MVB, and later degraded.
The EGFR can initiate signaling pathways that  lead to cell proliferation, migration, and differentiation. Overexpression of EGFR  stimulates cells to proliferate. Excessive  EGFR...
2.4K
MicroRNAs01:22

MicroRNAs

MicroRNA (miRNA) are short, regulatory RNA transcribed from introns—non-coding regions of a gene—or intergenic regions—stretches of DNA present between genes. Several processing steps are required to form biologically active, mature miRNA. The initial transcript, called primary miRNA (pri-mRNA), base-pairs with itself forming a stem-loop structure. Within the nucleus, an endonuclease enzyme, called Drosha, shortens the stem-loop structure into hairpin-shaped pre-miRNA. After...
22.6K