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Related Experiment Video

Updated: Oct 29, 2025

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis
08:48

Separation and Fractionation of Culture Filtrate Proteins (CFPs) from Mycobacterium tuberculosis

Published on: July 11, 2025

273

Methods for Proteomic Analyses of Mycobacteria.

Carolina Mehaffy1, Megan Lucas1, Nicole A Kruh-Garcia1,2

  • 1Mycobacteria Research Laboratories, Department of Microbiology, Immunology and Pathology, Colorado State University, Fort Collins, CO, USA.

Methods in Molecular Biology (Clifton, N.J.)
|July 8, 2021
PubMed
Summary

This chapter details mycobacterial protein sample processing for top-down and bottom-up proteomic analyses. It emphasizes critical steps for high-quality mass spectrometry data in Mycobacterium research.

Keywords:
Bottom-Up ProteomicsESI (Electro-Spray Ionization)LC-MS (Liquid Chromatography - Mass Spectrometry)MALDI-ToF (Matrix Assisted Laser Desorption Ionization - Time of Flight)MRM (Multiple Reaction Monitoring)PTM (Post-translational modification)Sample processingShotgun proteomicsTop-Down Proteomics

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Last Updated: Oct 29, 2025

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Area of Science:

  • Microbiology
  • Proteomics
  • Mass Spectrometry

Background:

  • Proteomic technologies offer insights into Mycobacterium spp. functions, diversity, and interactions.
  • Mass spectrometry is a key tool for microbial proteomics.
  • Effective study design and sample processing are crucial for reliable proteomic data.

Purpose of the Study:

  • To provide standardized protocols for processing mycobacterial protein samples.
  • To facilitate top-down and bottom-up proteomic analyses in Mycobacterium research.
  • To enhance the quality and reproducibility of mycobacterial proteomic studies.

Main Methods:

  • Development of specific sample processing protocols for mycobacteria.
  • Application of mass spectrometry-based proteomic techniques.
  • Integration of bioinformatics for data curation and statistical rigor.

Main Results:

  • Established protocols for preparing mycobacterial protein samples for proteomic analysis.
  • Demonstrated applicability of these protocols to both top-down and bottom-up mass spectrometry.
  • Highlighted the importance of organism-specific sample preparation.

Conclusions:

  • Standardized sample processing is essential for high-quality mycobacterial proteomics.
  • The provided protocols support diverse mass spectrometry approaches.
  • This work aids in advancing the understanding of Mycobacterium proteomes.