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Assessment of Mitochondrial Oxygen Consumption Using a Plate Reader-based Fluorescent Assay
Published on: April 12, 2024
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Mitochondria and oxygen homeostasis.
Mateus P Mori1, Rozhin Penjweini2, Jay R Knutson2
1Cardiovascular Branch, National Heart, Lung, and Blood Institute, National Institutes of Health, Bethesda, MD, USA.
The FEBS Journal
|July 8, 2021
Summary
Molecular oxygen is essential for life but can be toxic. This review explores how cells, particularly mitochondria, manage oxygen levels (oxygen homeostasis) to maintain health and prevent disease.
Area of Science:
- Biochemistry
- Cell Biology
- Physiology
Background:
- Molecular oxygen's dual role: essential for energy production yet a source of damaging reactive oxygen species.
- Aerobic life depends on finely tuned evolutionary mechanisms for cellular oxygen handling.
- Mitochondria play a central role in cellular oxygen consumption and homeostasis.
Purpose of the Study:
- To review the literature on oxygen homeostasis mechanisms.
- To highlight the mitochondrion's critical role in oxygen management.
- To connect oxygen homeostasis to disease pathogenesis and health maintenance.
Main Methods:
- Literature review of in vitro and in vivo studies on oxygen homeostasis.
- Focus on mitochondrial function and oxygen consumption.
- Analysis of evolutionary adaptations for oxygen handling.
Main Results:
- Oxygen homeostasis involves complex, evolutionarily conserved mechanisms.
- Mitochondria are key regulators of cellular oxygen levels.
- Dysregulation of oxygen homeostasis is linked to disease.
Conclusions:
- Understanding oxygen homeostasis is crucial for preventing and treating diseases.
- Mitochondrial function is central to maintaining cellular health in an oxygen-rich environment.
- Basic research into oxygen management provides insights for therapeutic strategies.
Keywords:
altitudecancercardiovascularhypoxialifespanmetabolismmitochondriaoxidative stressoxygenoxygen toxicitytissue oxygenMore Related Videos
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