[Detection of anti-DNA antibodies using I125-protein A]

Our purpose is to detect and dose anti-DNA antibodies in sera with analytical interferences in compatibility tests. This method seems with interest to clear transfusional reactions or incompatibilities in the laboratory tests. With 122 sera presenting a minor and isolated incompatibility with bromelin, we've found 21 positive results correlated or not (18/26) with the IF. The exposed methodology, simple, fast and reproducible seems to be a useful tool to resolve some transfusional situations.