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[Functional adjuvant for stored red blood cells: ascorbate or oxalate?]
G Ulrich1, M Allary, J Saint-Blancard
1Centre de Transfusion Sanguine des Armées Jean Julliard, Clamart.
Revue Francaise De Transfusion Et Immuno-Hematologie
|October 1, 1987
Summary
The study found that impurities in additives like ascorbate-2-phosphate (AsP) actually enhance their effectiveness in preserving stored blood cells. Oxalate, an impurity, showed promising results, challenging the direct efficacy of ascorbate itself.
Area of Science:
- Biochemistry
- Hematology
- Blood Preservation
Context:
- Stored blood cells experience rapid decreases in p50 and 2,3-DPG concentrations.
- This leads to functional weakness in transfused blood.
- Current additives like ascorbate and ascorbate-2-phosphate (AsP) have limitations in stability and efficacy.
Purpose:
- To investigate the effectiveness of commercially available ascorbate-2-phosphate (AsP) preparations.
- To determine the role of impurities in the efficacy of AsP for blood preservation.
- To explore alternative compounds, such as oxalate, for improving stored blood cell function.
Summary:
- Two commercially available AsP products with varying impurity levels were tested.
- Higher impurity content correlated with greater effectiveness in preserving blood cell function.
- Experiments with oxalate, identified as a key impurity, yielded results comparable to those previously attributed to AsP.
Impact:
- Challenges the perceived efficacy of pure ascorbate in blood preservation.
- Suggests that impurities, specifically oxalate, may be the active agents responsible for functional improvements in stored blood.
- Opens new avenues for developing more effective blood storage additives by focusing on specific compounds rather than solely on primary additives.