[Progress in PiggyBac transposition system research and its application in the gene modified T cell in tumor...

The tumor immunotherapy with gene modified T cells has made inspiring progress in recent years. Foreign gene transduction is the key link before the adoptive transfer of T cells. Advances in this field enhance T cells vitality and achieve precise targeted killing of tumor cells. T cell gene modification technology needs to strike a balance between transduction efficiency, safety, and cost. Transposons are with low immunogenicity, easy to manufacture, and cost-effective. They integrate the gene of interest into the target cell genome and allow stable and durable expression. Active transposons in mammalian cells include PiggyBac (PB), Sleeping Beauty (SB) and Tol2. Among them, the PB transposition system enables seamless genome editing with large gene load, high transposition activity, and strong structural plasticity. This article summarizes the structure, mechanism, characteristics, and transduction efficiency of PB transposition system, as well as its application and prospect in chimeric antigen receptor-engineered T cell therapy.