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Microfluidic origami nano-aptasensor for peanut allergen Ara h1 detection
Hai Jiang1, Qian Guo2, Cheng Zhang2
1School of Mechanical and Electrical Engineering, University of Electronic Science and Technology of China, Chengdu, Sichuan 611731, China; Institute of Electronic and Information Engineering of UESTC in Guangdong, Dongguan, Guangdong 523808, China.
A novel origami microfluidic aptasensor rapidly detects the peanut allergen Ara h 1 using aptamer-decorated black phosphorus nanosheets. This point-of-need sensor offers a faster alternative to traditional lab testing for food allergen analysis.
Area of Science:
- * Analytical Chemistry
- * Materials Science
- * Food Science
Background:
- * Accurate detection of food allergens like peanut Ara h 1 is crucial for public health.
- * Existing methods often require complex laboratory equipment and time-consuming procedures.
- * There is a need for rapid, sensitive, and portable diagnostic tools for on-site allergen detection.
Purpose of the Study:
- * To develop a novel origami microfluidic electrochemical nano-aptasensor for rapid Ara h 1 detection.
- * To utilize black phosphorus nanosheets (BPNSs) for enhanced sensing performance.
- * To provide a point-of-need analytical tool for food allergen analysis.
Main Methods:
- * Fabrication of a paper-based microfluidic device via sequential folding of patterned chromatography paper.
- * Screen-printed electrodes were integrated onto the paper substrate.
- * Aptamer-decorated BPNSs were electrodeposited as recognition elements for electrochemical detection.
Main Results:
- * The aptasensor achieved detection within 20 minutes.
- * A linear detection range of 50–1000 ng/mL for Ara h 1 was established.
- * A low detection limit of 21.6 ng/mL was achieved, with successful detection in spiked cookie dough samples.
Conclusions:
- * The developed origami microfluidic aptasensor offers a rapid and sensitive method for Ara h 1 detection.
- * The integration of BPNSs enhances electrochemical signal amplification and sensor selectivity.
- * This technology bridges the gap between laboratory analysis and practical, on-site food allergen screening.
