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Related Experiment Video

Updated: Oct 29, 2025

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry
09:12

Measuring Deformability and Red Cell Heterogeneity in Blood by Ektacytometry

Published on: January 12, 2018

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Multi-volume hemacytometer.

Ravangnam Thunyaporn1, Il Doh2, Dong Woo Lee3,4

  • 1Department of Biomedical Engineering, Konyang University, Daejeon, 35365, Republic of Korea.

Scientific Reports
|July 9, 2021
PubMed
Summary
This summary is machine-generated.

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A novel multi-volume hemacytometer expands cell counting range, enabling accurate measurements from 5x10^3 to 1x10^6 cells/ml. This innovation reduces sample preparation and improves cell viability monitoring.

Area of Science:

  • * Cell Biology
  • * Biophysics
  • * Laboratory Instrumentation

Background:

  • * Cell counting is crucial for monitoring cell viability and proliferation.
  • * Standard hemacytometers use a fixed volume, limiting measurement range and requiring sample dilution or concentration adjustments.
  • * Current automated hemacytometers operate within a typical range of 10^5 to 10^6 cells/ml.

Purpose of the Study:

  • * To develop a new hemacytometer with a multi-volume chamber to overcome the limitations of fixed-volume devices.
  • * To enhance the measurable cell concentration range for improved cell counting accuracy.
  • * To reduce the need for sample re-preparation in cell counting assays.

Main Methods:

  • * Development of a novel hemacytometer featuring a multi-volume chamber with four distinct depths (0.1, 0.2, 0.4, and 0.8 µl).

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Last Updated: Oct 29, 2025

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  • * Implementation of a Multi-Volume Cell Counting model utilizing the slope value of cell counts across multiple chambers.
  • * Comparative analysis of the multi-volume hemacytometer against a standard fixed-volume hemacytometer.

    • Main Results:

    • * The multi-volume hemacytometer achieved a significantly wider measurement range, from 5x10^3 to 1x10^6 cells/ml.
    • * The minimum measurable cell concentration was reduced twenty-fold compared to a standard 0.1 µl hemacytometer.
    • * The Multi-Volume Cell Counting model reduced total cell counting numbers by 62.5% compared to a 0.8 µl chamber, enhancing efficiency.

    Conclusions:

    • * The developed multi-volume hemacytometer offers a broader and more sensitive range for cell concentration measurement.
    • * This innovation streamlines cell counting workflows by minimizing sample manipulation.
    • * The multi-volume design and counting model represent a significant advancement in cell counting technology for research and diagnostics.