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Labeling DNA Probes03:31

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DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
Radioisotopes, fluorophores, or small molecule binding partners like biotin or digoxigenin, are the most widely used reporter tags for labeling DNA probes. These labels can be attached to the probe DNA molecule via...
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Synthesis of Wavelength-shifting DNA Hybridization Probes by Using Photostable Cyanine Dyes
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Cyanine-based Fluorescent Probe for Cyanide Ion Detection.

Mahesh Gosi1, Nagaraju Marepu1, Yeturu Sunandamma2

  • 1Department of Chemistry, Acharya Nagarjuna University, Nagarjunanagar, Guntur-522510, Andhra Pradesh, India.

Journal of Fluorescence
|July 9, 2021
PubMed
Summary

A new cyanine-based probe was developed for sensitive cyanide ion detection. This probe exhibits a distinct color change and can be used in a simple test kit for real-time monitoring.

Keywords:
Cyanide ionFluorimetric probeNucleophilic additionOrange to colorlessTest strip

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Area of Science:

  • Chemical Sensing
  • Analytical Chemistry
  • Organic Synthesis

Background:

  • Cyanide ion (CN-) is a highly toxic substance requiring sensitive detection methods.
  • Existing cyanide detection methods can be complex or lack selectivity.
  • Development of novel probes for rapid and reliable cyanide detection is crucial.

Purpose of the Study:

  • To synthesize and characterize a novel cyanine-based probe for selective cyanide ion detection.
  • To evaluate the probe's visual and instrumental sensing capabilities.
  • To develop a practical test kit for real-time cyanide monitoring.

Main Methods:

  • Two-step synthesis of a cyanine-based probe featuring indolium iodide and indole units.
  • Visual detection of color change upon addition of various ions.
  • Instrumental analysis using UV-Vis and fluorescence spectroscopy.
  • Mechanism elucidation using proton nuclear magnetic resonance (1H-NMR) spectroscopy.
  • Preparation of a filter paper-based test kit.

Main Results:

  • The synthesized probe displayed a distinct color change from orange to colorless exclusively in the presence of cyanide ions.
  • No significant visual or spectral changes were observed with other tested ions, indicating high selectivity.
  • 1H-NMR studies confirmed the nucleophilic addition mechanism of cyanide to the probe.
  • The developed filter paper test kit demonstrated effective real-time cyanide detection with a visual color change.

Conclusions:

  • A novel cyanine-based probe was successfully synthesized for selective and sensitive cyanide ion detection.
  • The probe offers a simple, visual, and instrumental method for identifying cyanide ions.
  • The developed test kit provides a practical tool for on-site, real-time cyanide monitoring.