Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Urine Studies II: Urine Culture and Sensitivity Test01:26

Urine Studies II: Urine Culture and Sensitivity Test

257
A urine culture and sensitivity test is a diagnostic procedure used to identify urinary tract bacterial infections and determine the most effective antibiotics for treatment. This test is generally preferred when a patient shows manifestations of a urinary tract infection, such as frequent or painful urination, cloudy or foul-smelling urine, or lower abdominal pain.Purpose of the TestThe primary goals of a urine culture and sensitivity test are to:Determine the specific bacteria causing the...
257

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Spectrophotometric determination of LAP photoinitiator radicalization in ophthalmic applications.

Biomedical materials (Bristol, England)·2026
Same author

Seascape heterogeneity and predictability drive movement strategy selection in estuarine predators.

The Journal of animal ecology·2025
Same author

Evaluating urine volume and host depletion methods to enable genome-resolved metagenomics of the urobiome.

Microbiome·2025
Same author

Environment and weight class linked to skin microbiome structure of juvenile Eastern hellbenders (Cryptobranchus alleganiensis alleganiensis) in human care.

PloS one·2025
Same author

Are you my mother? When host genetics and gut microbiota tell different phylogenetic stories in the Africanized honey bee hybrid (<i>Apis mellifera scutellata</i> × sspp.).

Microbiology spectrum·2025
Same author

A case of multiple basal cell carcinomas in a patient with MUTYH gene mutation.

JAAD case reports·2025

Related Experiment Video

Updated: Oct 29, 2025

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

14.7K

Evaluating extraction methods to study canine urine microbiota.

Ryan Mrofchak1, Christopher Madden1, Morgan V Evans1,2

  • 1Department of Veterinary Preventive Medicine, Ohio State University College of Veterinary Medicine, Columbus, Ohio, United States of America.

Plos One
|July 9, 2021
PubMed
Summary

The Bacteremia Kit effectively extracts bacterial DNA from canine urine, crucial for studying the urinary microbiota. This method aids research into urogenital diseases in dogs and other species.

More Related Videos

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

606
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.2K

Related Experiment Videos

Last Updated: Oct 29, 2025

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples
07:24

A Hybrid DNA Extraction Method for the Qualitative and Quantitative Assessment of Bacterial Communities from Poultry Production Samples

Published on: December 10, 2014

14.7K
Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment
04:35

Saline Lavage for Sampling of the Canine Nasal Immune Microenvironment

Published on: December 27, 2024

606
Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction
09:19

Capturing Actively Produced Microbial Volatile Organic Compounds from Human-Associated Samples with Vacuum-Assisted Sorbent Extraction

Published on: June 1, 2022

4.2K

Area of Science:

  • Microbiology
  • Genomics
  • Veterinary Science

Background:

  • The urinary microbiota, microbes in urine, influences host health.
  • Traditional culture methods miss diverse urinary microbes.
  • Urine's low biomass and contamination risk necessitate sensitive DNA extraction.

Purpose of the Study:

  • To compare five commercial DNA extraction kits for canine urine.
  • To identify the most effective method for urinary microbiota analysis.
  • To establish a foundation for studying canine urogenital diseases.

Main Methods:

  • Collected urine from ten healthy dogs.
  • Extracted DNA using five different commercial kits.
  • Assessed DNA yield and bacterial community via 16S rRNA sequencing.

Main Results:

  • Bacteremia Kit yielded highest total and bacterial DNA concentrations.
  • Bacteremia Kit extracted DNA from the most samples.
  • Urinary microbiota varied significantly by dog and sex, not extraction method.

Conclusions:

  • The Bacteremia Kit is a suitable choice for canine urine microbiota studies.
  • This research supports further investigation of the canine urinary microbiome.
  • Findings contribute to understanding urogenital health and disease in dogs.