Design and performance of a hyperspectral camera for full-face in vivo imaging

Red, green, blue color photography is a mature technology and a powerful tool for the evaluation and understanding of the way an object reflects light and its related optical properties, but color photography fails to give a complete picture of these effects due to its inherent lack of spectral resolution. In this work, we update the L'OREAL reference device for skin color measurement, the Chromasphere, by replacing its current color camera system with an imaging spectrometer. This imaging spectrometer must provide a spatial resolution on par with the previous color cameras and a spectral resolution commensurate with a spectroradiometer while also achieving a time resolution suitable for in vivo studies of the human face. Due to these requirements, common spatial scanning techniques are not suitable for this application, and so we utilized a spectral-scanning approach based on a tunable liquid-crystal birefringent filter. We present the design and performance tests of a working prototype that is capable of measuring the spectrum in each of 4 MP with a nominal spectral resolution of 10 nm across the wavelength range from 420 to 730 nm in a total imaging time of less than 10 s. We cross-compared the spectral and color measurements obtained with this prototype, an industry-standard spectroradiometer, and a charge-coupled device color camera in order to assess the prototype's performance, and the results of this comparison show that our prototype is capable of taking spectral measurements near enough in quality to those of a spectroradiometer to successfully bridge the divide between such devices and conventional color cameras. Doing so, this instrument opens new possibilities for studies of complex in vivo phenomena that neither non-imaging spectrometers nor conventional cameras can pursue.