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Updated: Oct 29, 2025

A Flame-Free Method for Sterilizing C. elegans Picks, Spatulas, and Scalpels
Published on: March 4, 2022
Forced convective heating for low-temperature sterilization
Seung Hun Lee1, Hyun Jeong Jeon1, Youbong Lim1
1Plasmapp Co., Ltd., 372 Dongbu-daero, Osan-si, Gyeonggi-do 18151, Republic of Korea.
Abstract:
A novel impermeable sterile pouch is developed to allow the forced convective heating mechanism for improving the sterilization cycle. The heating process is parametrically investigated to obtain an optimized condition in which a sterilization load is heated from 20 to 45 °C within 2 min, and the forced convection is experimentally and numerically analyzed to find that the convection coefficient is dramatically increased to 450 W/m2 K when compared with the conventional natural convection coefficient of 80 W/m2 K. The optimized heating process is applied to a sterilization cycle using the impermeable pouch, and the overall sterilization cycle is found to be completed within 7.5 min whose performance is validated by using a process challenge device.
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