Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

M-K medium and postmortem cytologic damage.

P S Binder, M G Wickham

    Investigative Ophthalmology & Visual Science
    |February 1, 1978
    PubMed
    Summary
    This summary is machine-generated.

    M-K medium storage preserves corneal endothelium viability for up to 48 hours, extending the usability of donor corneas for transplantation. This method shows promise for corneas with extended post-mortem preservation times.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Concomitant chemotherapy and radiation for the treatment of advanced-stage endometrial cancer.

    Gynecologic oncology·2014
    Same author

    New method for reshaping the cornea.

    Journal of biomedical optics·2012
    Same author

    Loop-mirror filters based on saturable-gain or-absorber gratings.

    Optics letters·2007
    Same author

    A new surgical technique for deep stromal, anterior lamellar keratoplasty.

    The British journal of ophthalmology·1999
    Same author

    Posterior lamellar keratoplasty for a case of pseudophakic bullous keratopathy.

    American journal of ophthalmology·1999
    Same author

    Topography following keratoplasty in cats is not representative of that in humans.

    Journal of refractive surgery (Thorofare, N.J. : 1995)·1999
    Same journal

    Impact of Subretinal Drusenoid Deposits on Ellipsoid Zone-Related Thickness Metrics.

    Investigative ophthalmology & visual science·2026
    Same journal

    Proteomic Profiling of Optic Nerves From SMOX-Deficient Mice Identifies Regulators of Neuroinflammation and Axonal Damage in Optic Neuritis.

    Investigative ophthalmology & visual science·2026
    Same journal

    Aflibercept and Faricimab Equipotently Restore Endothelial Barrier Function.

    Investigative ophthalmology & visual science·2026
    Same journal

    Spatial Decomposition of Longitudinal RNFL Maps Reveals Distinct Modes of Glaucomatous Progression With Structure-Function and Genetic Signatures.

    Investigative ophthalmology & visual science·2026
    Same journal

    The CXXC1-IGFBP6 Axis Maintains Corneal Epithelial Differentiation via H3K4me3-Dependent Transcriptional Activation.

    Investigative ophthalmology & visual science·2026
    Same journal

    Archetypal Visual Field Analysis of Patients With Chronic Leber Hereditary Optic Neuropathy in Relation to Visual Recovery.

    Investigative ophthalmology & visual science·2026
    See all related articles

    Area of Science:

    • Ophthalmology
    • Transplantation Biology
    • Cell Biology

    Background:

    • Corneal transplantation relies on donor tissue viability.
    • Standard eye bank storage has limitations on preservation time.
    • Optimizing preservation methods is crucial for increasing donor cornea availability.

    Purpose of the Study:

    • To evaluate the efficacy of M-K medium in preserving corneal endothelial cell morphology and function.
    • To determine the optimal storage duration of corneas in M-K medium after initial refrigeration.
    • To assess the potential of M-K medium for extending the viability of donor corneas for transplantation.

    Main Methods:

    • Rabbit eyes were refrigerated, and corneas were stored in M-K medium at +4°C for varying durations (18, 24, 48 hours).

    Related Experiment Videos

  • Endothelial cell viability was assessed using nitroblue tetrazolium and microscopy (light, SEM, TEM).
  • Corneas were used in allotransplantation models, with recipient evaluation via slit-lamp biomicroscopy and pachometry.

    • Main Results:

    • M-K medium storage maintained corneal endothelial cell integrity up to 48 hours for corneas stored initially for 2, 6, and 9 days.
    • No significant protective effect of M-K medium was observed for corneas stored initially for 21 days.
    • Transplanted corneas stored in M-K medium showed preserved morphology and function compared to controls.

    Conclusions:

    • M-K medium effectively extends the preservation time of corneal endothelium for up to 48 hours beyond standard moist chamber storage.
    • This extended preservation is most effective for corneas with shorter initial post-mortem to storage intervals.
    • M-K medium holds potential for increasing the utilization of donor corneas, particularly those with moderately prolonged post-mortem intervals.