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Effect of incubation time and temperature on the interference of digoxin-like immunoreactive substances in digoxin
L Yannakou1, E P Diamandis, A Souvatzoglou
1Department of Therapeutics, Faculty of Medicine, University of Athens, Alexandra Hospital, Greece.
We have tested the cross-reactivity of digoxin-like immunoreactive substances (DLIS) of cord serum in three commercially available radioimmunoassay systems. We found that there is a marked difference in the specificity of the three antibodies. We were able to eliminate or greatly reduce the interference of DLIS by incubating for 1 h at 37 degrees C. The degree of interference for various cord blood sera is given at three temperature levels and at incubation times ranging from 15 min to 48 h.
We have tested the cross-reactivity of digoxin-like immunoreactive substances (DLIS) of cord serum in three commercially available radioimmunoassay systems. We found that there is a marked difference in the specificity of the three antibodies. We were able to eliminate or greatly reduce the interference of DLIS by incubating for 1 h at 37 degrees C. The degree of interference for various cord blood sera is given at three temperature levels and at incubation times ranging from 15 min to 48 h.