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Components and Functional Diversification of Florigen Activation Complexes in Cotton
Hui Liu1, Xianzhong Huang2,3, Bin Ma1
1College of Life Sciences, Shihezi University, Shihezi 832003, China.
Plant & Cell Physiology
|July 10, 2021
Summary
Cotton
Area of Science:
- Plant Molecular Biology
- Plant Development
- Genetics and Genomics
Background:
- Florigen Activation Complex (FAC) regulates flowering in plants.
- Cotton (Gossypium hirsutum L.) has a single FT locus in its A- and D-subgenomes.
- Limited information exists on cotton FACs and their functions.
Purpose of the Study:
- To identify and characterize cotton 14-3-3 and FD proteins.
- To investigate the interactions within the cotton FAC.
- To determine the roles of GhFD genes in cotton development.
Main Methods:
- Protein interaction assays (in vivo and in vitro).
- Virus-induced gene silencing (VIGS) in cotton.
- Gene expression analysis and transcriptional activation assays.
- Heterologous expression in Arabidopsis.
Main Results:
- Identified a cotton 14-3-3 protein interacting with GhFT.
- Characterized five GhFD homoeologous gene pairs, all interacting with Gh14-3-3 and GhFT to form a ternary complex.
- Silencing GhFD1, -2, and -4 delayed flowering; silencing GhFD3 impaired lateral root formation.
- GhFD1 and GhFD2 promote flowering with functional redundancy; GhFD3 activates auxin response factor genes, crucial for lateral root development.
Conclusions:
- Cotton FACs are formed by GhFT, Gh14-3-3, and GhFD proteins.
- Different GhFD members exhibit diverse functions, regulating flowering and lateral root development.
- Targeted genome editing of the florigen system could optimize cotton flowering and architecture.
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