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Completing the canvas: advances and challenges for DNA-PAINT super-resolution imaging
Raman van Wee1, Mike Filius1, Chirlmin Joo1
1Department of BioNanoScience, Kavli Institute of Nanoscience, Delft University of Technology, Van der Maasweg 9, 2629 HZ Delft, The Netherlands.
Trends in Biochemical Sciences
|July 12, 2021
Summary
DNA-PAINT, a super-resolution microscopy technique, uses DNA probes for high-resolution imaging. Recent advances are overcoming limitations, paving the way for live-cell applications.
Area of Science:
- Biophysics
- Molecular Imaging
- Cell Biology
Background:
- Single-molecule localization microscopy (SMLM) offers high resolution for biological studies.
- DNA-PAINT utilizes transient DNA probe binding for nanoscale imaging.
- Early DNA-PAINT faced challenges in throughput, speed, and live-cell compatibility.
Purpose of the Study:
- To review the current advancements in DNA-PAINT technology.
- To discuss how recent developments address previous limitations.
- To identify future needs for live-cell DNA-PAINT imaging.
Main Methods:
- Exploitation of stochastic and transient binding of fluorescently labeled DNA probes.
- Advancements in SMLM techniques.
- Analysis of current DNA-PAINT implementations and their improvements.
Main Results:
- DNA-PAINT has evolved significantly, overcoming initial hurdles.
- New strategies are enhancing throughput and reducing acquisition times.
- Progress is being made towards integrating DNA-PAINT with live-cell imaging.
Conclusions:
- Recent advances are making DNA-PAINT more practical and versatile.
- Further developments are crucial for realizing the full potential of live-cell DNA-PAINT.
- The technique is poised to expand its applications in biological research.
