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Comprehensive Analysis of the Transcriptome-Wide m6A Methylome in Pterygium by MeRIP Sequencing
Yaping Jiang1, Xin Zhang1, Xiaoyan Zhang2
1Department of Ophthalmology, Yangpu Hospital, Tongji University School of Medicine, Shanghai, China.
Frontiers in Cell and Developmental Biology
|July 12, 2021
Summary
N6-methyladenosine (m6A) modification is altered in pterygium, a common eye condition. This study identifies key genes and pathways involved, suggesting m6A as a potential therapeutic target for pterygium.
Area of Science:
- Ophthalmology
- Molecular Biology
- Epigenetics
Background:
- Pterygium is a prevalent ocular surface disease that can lead to vision impairment.
- N6-methyladenosine (m6A) is a crucial mRNA modification regulating gene expression, but its role in pterygium is unexplored.
Purpose of the Study:
- To investigate the role of m6A modification in the pathogenesis of pterygium.
- To identify specific genes and pathways affected by m6A dysregulation in pterygium.
Main Methods:
- Compared m6A modification levels in pterygium and normal conjunctival tissues using an m6A RNA Methylation Quantification Kit.
- Analyzed the expression and localization of METTL3, an m6A methyltransferase.
- Utilized m6A-RNA immunoprecipitation sequencing (MeRIP-seq) and RNA sequencing (RNA-seq) with bioinformatics analysis.
Main Results:
- Identified 2,949 dysregulated m6A peaks in pterygium tissue, with 2,145 upregulated and 804 downregulated.
- Found altered m6A peaks in genes associated with the Hippo signaling pathway and endocytosis.
- Identified five key genes (DSP, MXRA5, ARHGAP35, TMEM43, OLFML2A) linked to pterygium development through integrated analysis.
Conclusions:
- m6A modification is significantly dysregulated in pterygium development.
- The findings highlight m6A as a potential therapeutic target for pterygium treatment.
