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Related Experiment Videos

V-factor dependent strains of Pasteurella multocida subsp. multocida.

T Krause1, H U Bertschinger, L Corboz

  • 1Institut für Medizinische Mikrobiologie der Philipps-Universität, Marburg, Federal Republic of Germany.

Zentralblatt Fur Bakteriologie, Mikrobiologie, Und Hygiene. Series A, Medical Microbiology, Infectious Diseases, Virology, Parasitology
|August 1, 1987
PubMed
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Two V-factor requiring bacterial strains were isolated from swine lungs with Mycoplasma pneumoniae. DNA hybridization confirmed these isolates as Pasteurella multocida subsp. multocida, with preliminary V-factor requirement studies conducted.

Area of Science:

  • Veterinary Microbiology
  • Bacteriology
  • Animal Pathology

Background:

  • Swine pneumonia is often caused by Mycoplasma hyopneumoniae.
  • Bacterial co-infections can complicate respiratory disease in swine.
  • Pyridine nucleotide (V-factor) is essential for the growth of certain bacteria.

Purpose of the Study:

  • To identify and characterize V-factor requiring bacteria isolated from swine lungs with Mycoplasma hyopneumoniae-induced pneumonia.
  • To determine if these isolates belong to the Pasteurella multocida species.
  • To conduct preliminary investigations into their V-factor nutritional requirements.

Main Methods:

  • Bacteriological examination of swine lung tissues.
  • Biochemical characterization of isolated bacterial strains.

Related Experiment Videos

  • Deoxyribonucleic acid (DNA)-DNA hybridization for species identification.
  • Preliminary analysis of V-factor requirements.

    • Main Results:

    • Two strains of V-factor requiring bacteria were isolated.
    • Biochemical profiles were consistent with Pasteurella multocida subsp. multocida.
    • DNA-DNA hybridization confirmed the isolates as V-factor requiring strains of Pasteurella multocida subsp. multocida.
    • Initial data on V-factor requirements were obtained.

    Conclusions:

    • V-factor requiring Pasteurella multocida subsp. multocida can be isolated from swine lungs affected by Mycoplasma hyopneumoniae.
    • These findings highlight potential bacterial co-pathogens in swine respiratory disease.
    • Further research into the specific V-factor needs of these strains is warranted.