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V-factor dependent strains of Pasteurella multocida subsp. multocida
T Krause1, H U Bertschinger, L Corboz
1Institut für Medizinische Mikrobiologie der Philipps-Universität, Marburg, Federal Republic of Germany.
Abstract:
At bacteriological examination of swine lungs with pneumoniae due to Mycoplasma hyopneumoniae, two strains of pyridine nucleotide (V-factor) requiring bacteria were isolated. They exhibited a pattern of biochemical reactions fitting with that of Pasteurella multocida subsp. multocida. Deoxyribonucleic acid (DNA): DNA hybridization with these isolates and Pasteurella multocida subsp. multocida showed that they were V-factor requiring strains of this species. Preliminary investigations on the qualitative and quantitative V-factor requirements have been performed.
Insights
Two V-factor requiring bacterial strains were isolated from swine lungs with Mycoplasma pneumoniae. DNA hybridization confirmed these isolates as Pasteurella multocida subsp. multocida, with preliminary V-factor requirement studies conducted.
Area of Science:
- Veterinary Microbiology
- Bacteriology
- Animal Pathology
Background:
- Swine pneumonia is often caused by Mycoplasma hyopneumoniae.
- Bacterial co-infections can complicate respiratory disease in swine.
- Pyridine nucleotide (V-factor) is essential for the growth of certain bacteria.
Purpose of the Study:
- To identify and characterize V-factor requiring bacteria isolated from swine lungs with Mycoplasma hyopneumoniae-induced pneumonia.
- To determine if these isolates belong to the Pasteurella multocida species.
- To conduct preliminary investigations into their V-factor nutritional requirements.
Main Methods:
- Bacteriological examination of swine lung tissues.
- Biochemical characterization of isolated bacterial strains.
- Deoxyribonucleic acid (DNA)-DNA hybridization for species identification.
- Preliminary analysis of V-factor requirements.
Main Results:
- Two strains of V-factor requiring bacteria were isolated.
- Biochemical profiles were consistent with Pasteurella multocida subsp. multocida.
- DNA-DNA hybridization confirmed the isolates as V-factor requiring strains of Pasteurella multocida subsp. multocida.
- Initial data on V-factor requirements were obtained.
Conclusions:
- V-factor requiring Pasteurella multocida subsp. multocida can be isolated from swine lungs affected by Mycoplasma hyopneumoniae.
- These findings highlight potential bacterial co-pathogens in swine respiratory disease.
- Further research into the specific V-factor needs of these strains is warranted.