Advances in static in vitro digestion models after the COST action Infogest consensus protocol

Area of Science:

• Food Science
• Biochemistry
• Nutritional Science

Background:

• In vitro digestion models are crucial for predicting food molecule bioaccessibility and bioactivity.
• Dynamic models mimic gastrointestinal conditions but face reproducibility challenges.
• Static models offer a simpler, validated alternative applicable across nutritional, pharmaceutical, and toxicological fields.

Purpose of the Study:

• To review the impact of the COST Action Infogest consensus method on static in vitro digestion models.
• To highlight the evolution of static digestion methods post-Infogest.
• To focus on the application of these methods in food science.

Main Methods:

• Review of scientific literature focusing on static in vitro digestion models.
• Analysis of the Infogest consensus method and its subsequent developments.
• Examination of studies utilizing static models for food applications.

Main Results:

• The Infogest consensus method (2014) significantly harmonized static digestion protocols.
• Standardization has improved the reproducibility and comparability of in vitro digestion studies.
• Static models have seen widespread adoption and refinement for food analysis.

Conclusions:

• The Infogest method has been pivotal in advancing static in vitro digestion techniques.
• These standardized methods are essential for reliable bioaccessibility and bioactivity assessments in food.
• Continued evolution of static models promises further insights into food-molecule interactions.