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Related Experiment Video

Updated: Oct 29, 2025

OnePot PURE Cell-Free System
08:25

OnePot PURE Cell-Free System

Published on: June 23, 2021

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OnePot PURE Cell-Free System.

Laura Grasemann1, Barbora Lavickova1, M Carolina Elizondo-Cantú1

  • 1Institute of Bioengineering, School of Engineering, École Polytechnique Fédérale de Lausanne.

Journal of Visualized Experiments : Jove
|July 12, 2021
PubMed
Summary
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Researchers developed OnePot PURE, a cost-effective method for producing cell-free protein synthesis systems. This streamlined approach makes synthetic biology more accessible by reducing preparation time and cost.

Area of Science:

  • Synthetic Biology
  • Biochemistry
  • Molecular Biology

Background:

  • The PURE (protein synthesis using recombinant elements) system is a valuable tool for cell-free synthetic biology.
  • Commercial PURE systems are expensive and lack tunability.
  • Home-made PURE systems are customizable but require extensive time and labor for component purification.

Purpose of the Study:

  • To develop an easy, adjustable, time- and cost-effective method for producing all PURE system components.
  • To streamline the preparation of home-made PURE systems by simplifying protein purification.
  • To expand the accessibility of PURE systems to a broader range of laboratories.

Main Methods:

  • Developed a streamlined protocol for producing all PURE system components within one week.

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  • Utilized coculturing and co-purification techniques to minimize labor and time.
  • Employed standard laboratory equipment for component preparation.

    • Main Results:

    • Successfully produced all necessary PURE system components in under a week.
    • The OnePot PURE system demonstrates performance comparable to commercial systems.
    • The method significantly reduces the time and cost associated with preparing PURE systems.

    Conclusions:

    • The OnePot PURE preparation method offers a simple, cost-effective, and efficient alternative to commercial systems.
    • This approach enhances the accessibility of cell-free synthetic biology platforms.
    • The streamlined method facilitates broader adoption of the PURE system in research settings.