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Microfluidic Applications for Disposable Diagnostics

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Published on: February 3, 2008

Portable Pathogen Diagnostics Using Microfluidic Cartridges Made from Continuous Liquid Interface Production Additive

Jacob Berger^1,2, Mehmet Y Aydin³, Robert Stavins³

¹Department of Bioengineering, University of Illinois Urbana-Champaign, Urbana, Illinois 61801, United States.

Analytical Chemistry

|July 12, 2021

Summary

Additive manufacturing using CLIP technology enables rapid production of microfluidic diagnostic cartridges. This innovation supports sensitive pathogen detection for point-of-care infectious disease diagnostics.

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Published on: November 21, 2023

Area of Science:

Biomedical Engineering
Additive Manufacturing
Microfluidics

Background:

Microfluidic devices offer significant potential for biomedical diagnostics, but manufacturing limitations hinder widespread adoption.
Infectious disease outbreaks highlight the need for simple, sensitive, and point-of-care diagnostic tests.
Conventional microfluidic device manufacturing methods face challenges in scalability and cost-effectiveness.

Purpose of the Study:

To develop and characterize additive manufacturing (AM) cartridges using Continuous Liquid Interface Production (CLIP) for microfluidic diagnostics.
To investigate the process capabilities and material compatibility of CLIP for fabricating microfluidic devices.
To validate the use of CLIP-based microfluidic cartridges for pathogen detection.

Main Methods:

Fabrication of microfluidic cartridges using CLIP, a form of additive manufacturing.
Characterization of microfluidic channel dimensions and repeatability produced by CLIP.
Development and implementation of a loop-mediated isothermal amplification (LAMP) assay for *E. coli* detection on the cartridges.

Main Results:

CLIP technology accurately produced microfluidic channels as small as 400 μm, with routine production of 100 μm channels at high repeatability.
A LAMP assay demonstrated a limit of detection of 50 cfu/μL for *E. coli* in whole blood directly on the CLIP-fabricated cartridges.
The study validated CLIP processes and materials for pathogen detection in microfluidic diagnostic devices.

Conclusions:

Continuous Liquid Interface Production (CLIP) is a viable additive manufacturing method for producing microfluidic diagnostic cartridges.
CLIP-based microfluidic devices enable sensitive and repeatable pathogen detection, suitable for point-of-care applications.
This portable diagnostic platform technology can be scaled for current and future infectious disease diagnostics and pandemic preparedness.