Edge-Enhanced Microwell Immunoassay for Highly Sensitive Protein Detection
Qingxuan Li1, Sidi A Bencherif1,2, Ming Su1
1Department of Chemical Engineering, Northeastern University, Boston, Massachusetts 02115, United States.
Analytical Chemistry
|July 12, 2021
Summary
This study introduces a novel edge enhancement technique using microwells for ultra-sensitive protein detection. This biosensor platform improves sensitivity for early disease diagnosis and single-cell analysis.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Biotechnology
Background:
- Highly sensitive biosensors are crucial for early disease diagnosis and treatment assessment.
- Detecting low concentrations of protein biomarkers and single-cell secretions requires advanced detection methods.
Purpose of the Study:
- To develop a novel signal amplification mechanism for ultra-sensitive protein detection.
- To utilize the edge enhancement effect of microwells for improved biosensing capabilities.
Main Methods:
- A new signal amplification mechanism based on the vertical sidewalls of microwells was developed.
- The edge enhancement effect, creating a microring effect, was leveraged for fluorescence signal amplification.
- Bovine serum albumin (BSA) detection was performed to calibrate the enhanced fluorescence intensity.
Main Results:
- A 6-fold sensitivity enhancement was observed at the microwell edge compared to a flat surface.
- A lower limit of detection was achieved at the microwell edge.
- The microwell chip successfully separated single cells and detected interferon-γ secretion from T cells.
Conclusions:
- The edge-enhancement ability of microwells provides a highly sensitive biosensing platform.
- This technique is suitable for early-stage disease diagnosis and single-cell level treatment assessment.
