The behaviour of corneal epithelium following a standardized alkali wound

The short and long-term healing of the rabbit corneal epithelium was studied after a standardized alkali wound. The wound was inflicted by applying a round filter paper, 5.5 mm in diameter, soaked in 1 N NaOH, for 60 seconds on the central cornea. The wound size and intensity was chosen not to cause melting and perforation, and not to cause vascular ingrowth. n-Heptanol corneal wounds of the same size were used as control. The eyes were followed for 8 weeks. Two phases of epithelial healing were discerned. The initial healing phase lasted 48 h during which the would was completely resurfaced. In spite of the more extensive tissue damage caused by alkali, the initial epithelial healing rate was faster than in n-heptanol wounds. The late healing phase consisted of recurrent epithelial break down, sometimes seen preceded by epithelial blister formation. Four weeks after trauma the state of epithelial healing was at its worst.