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miR-30a-5p suppresses lung squamous cell carcinoma via ATG5 - mediated autophagy

Jichen Yang1, Sunyin Rao1, Run Cao1

  • 1Department of Thoracic Surgery, Lung Cancer Research Center, Yunnan Institute of Oncology, the Third Affiliated Hospital of Kunming Medical University, Yunnan Cancer Hospital, Kunming Yunnan 650118, PR China.

Aging
|July 13, 2021
PubMed

Insights

MicroRNA-30a-5p inhibits lung squamous cell carcinoma (LUSC) progression by suppressing ATG5-mediated autophagy. This finding highlights a potential therapeutic target for LUSC treatment.

Area of Science:

  • Oncology
  • Molecular Biology
  • Cellular Biology

Background:

  • Autophagy plays a complex role in cancer development.
  • Understanding the specific mechanisms of autophagy in lung squamous cell carcinoma (LUSC) is crucial for targeted therapies.

Purpose of the Study:

  • To investigate the role of ATG5-induced autophagy in LUSC progression.
  • To elucidate the upstream regulatory mechanisms controlling ATG5 in LUSC.

Main Methods:

  • Analysis of TCGA database for differentially expressed miRNAs and mRNAs in LUSC.
  • RT-PCR and Western blot to assess autophagy gene expression.
  • Transmission electron microscopy and immunofluorescence for autophagy flux.
  • Dual-luciferase reporter assay to confirm miRNA-mRNA binding.
  • In vivo lung metastatic model to evaluate therapeutic effects.

Main Results:

  • High ATG5 expression was observed in LUSC patients.
  • Silencing ATG5 reduced autophagy flux in LUSC cells.
  • hsa-mir-30a-5p was identified as a direct binding partner of ATG5.
  • miR-30a-5p mimic suppressed ATG5-mediated autophagy and LUSC cell progression in vitro and in vivo.

Conclusions:

  • miR-30a-5p inhibits LUSC progression by targeting ATG5-mediated autophagy.
  • This study reveals a novel regulatory pathway with therapeutic implications for LUSC.
Keywords:
ATG5autophagylung squamous cell carcinomamiR-30a-5pprognosis

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