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Identification of Enterobacteriaceae by the API 20E system
Journal of Clinical Pathology
|January 1, 1978
Summary
This study evaluated identification schemes for the API 20E kit, finding the advanced Analytical Profile Index correctly identified 88% of Enterobacteriaceae strains. Results suggest modifying kit tests could improve bacterial identification accuracy.
Area of Science:
- Clinical Microbiology
- Bacterial Identification
- Diagnostic Performance
Background:
- The API 20E kit is a widely used tool for bacterial identification.
- Manufacturer-developed identification schemes aim to optimize API 20E kit performance.
- Evaluating these schemes is crucial for accurate bacterial classification.
Purpose of the Study:
- To assess the efficacy of various API 20E kit identification schemes.
- To compare the diagnostic performance of the API 20E kit against conventional methods.
- To identify potential improvements for the API 20E kit's identification capabilities.
Main Methods:
- Evaluation of multiple API 20E kit identification schemes using 206 Enterobacteriaceae strains.
- Comparison with a conventional identification system (50 tests and computer program).
- Separate assessment of API 20E kit tests and 52 conventional tests for taxonomic discrimination.
Main Results:
- The most advanced scheme, Analytical Profile Index, achieved 88% correct identification for 206 strains.
- Atypical strains presented challenges, with the conventional system identifying only 94%.
- 2% of strains were misidentified by the Analytical Profile Index scheme.
Conclusions:
- Current API 20E kit identification schemes show good, but not perfect, performance.
- The diagnostic accuracy of the API 20E kit may be enhanced by modifying specific tests.
- Further research into test optimization could improve Enterobacteriaceae identification.