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Identification of Enterobacteriaceae by the API 20E system.

B Holmes, W R Willcox, S P Lapage

Journal of Clinical Pathology

|January 1, 1978

Summary

This study evaluated identification schemes for the API 20E kit, finding the advanced Analytical Profile Index correctly identified 88% of Enterobacteriaceae strains. Results suggest modifying kit tests could improve bacterial identification accuracy.

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Area of Science:

Clinical Microbiology
Bacterial Identification
Diagnostic Performance

Background:

The API 20E kit is a widely used tool for bacterial identification.
Manufacturer-developed identification schemes aim to optimize API 20E kit performance.
Evaluating these schemes is crucial for accurate bacterial classification.

Purpose of the Study:

To assess the efficacy of various API 20E kit identification schemes.
To compare the diagnostic performance of the API 20E kit against conventional methods.
To identify potential improvements for the API 20E kit's identification capabilities.

Main Methods:

Evaluation of multiple API 20E kit identification schemes using 206 Enterobacteriaceae strains.
Comparison with a conventional identification system (50 tests and computer program).
Separate assessment of API 20E kit tests and 52 conventional tests for taxonomic discrimination.

Main Results:

The most advanced scheme, Analytical Profile Index, achieved 88% correct identification for 206 strains.
Atypical strains presented challenges, with the conventional system identifying only 94%.
2% of strains were misidentified by the Analytical Profile Index scheme.

Conclusions:

Current API 20E kit identification schemes show good, but not perfect, performance.
The diagnostic accuracy of the API 20E kit may be enhanced by modifying specific tests.
Further research into test optimization could improve Enterobacteriaceae identification.

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