Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Open Angle Glaucoma: Treatment

Open Angle Glaucoma: Treatment

In open-angle glaucoma, the iridocorneal angle remains open, but the trabecular meshwork becomes stiff, slowing down the outflow of aqueous humor. This causes a buildup of aqueous humor in the anterior chamber, leading to a sudden increase in intraocular pressure. The treatment for open-angle glaucoma focuses on reducing the elevated intraocular pressure by either decreasing the secretion of aqueous humor or increasing its outflow.
Drugs such as carbonic anhydrase inhibitors, α2- and...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Survival after hypofractionated radiation versus standard radiation in glioblastoma by MGMT promoter methylation status and age: an analysis of the national cancer database.

Journal of neuro-oncology·2026

Same author

Patient-derived surgical samples reveal the cellular and molecular signatures of glioblastoma infiltration in distinct radiological zones.

Brain pathology (Zurich, Switzerland)·2026

Same author

Cookies, Chips, and Seeds: How Human Food Leftovers Influence Ant-Mediated Seed Removal.

Biology·2026

Same author

REPLY.

Ophthalmology·2026

Same author

The evolution of the neurosurgery residency system in North America. Part I. From the origins to the 1970s.

Neurocirugia·2026

Same author

Atrial Fibrillation and Cognitive Decline: A Systematic Review of Pathophysiological Mechanisms, Therapeutic Strategies, and Digital Health Technologies in Neuroprotection.

Journal of clinical medicine·2026

Search research articles

Related Experiment Video

Updated: Oct 29, 2025

Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres

Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres

Published on: February 2, 2015

Long-Term Decrease of Intraocular Pressure in Rats by Viral Delivery of miR-146a.

Coralia Luna¹, Megan Parker¹, Pratap Challa¹

¹Duke University Eye Center, Durham, NC, USA.

Translational Vision Science & Technology

|July 13, 2021

Summary

MicroRNA-146a (miR-146a) delivery reduced intraocular pressure (IOP) in rats. This gene therapy approach shows promise for glaucoma treatment without adverse effects.

More Related Videos

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Published on: February 15, 2022

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

Related Experiment Videos

Last Updated: Oct 29, 2025

Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres

Experimental Glaucoma Induced by Ocular Injection of Magnetic Microspheres

Published on: February 2, 2015

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Full-Circle Cauterization of Limbal Vascular Plexus for Surgically Induced Glaucoma in Rodents

Published on: February 15, 2022

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

A Model of Glaucoma Induced by Circumlimbal Suture in Rats and Mice

Published on: October 5, 2018

Area of Science:

Ophthalmology
Molecular Biology
Gene Therapy

Background:

Glaucoma is a leading cause of irreversible blindness.
Elevated intraocular pressure (IOP) is a primary risk factor for glaucoma.
Trabecular meshwork (TM) dysfunction contributes to IOP elevation.

Purpose of the Study:

To investigate the role of microRNA-146a (miR-146a) in trabecular meshwork (TM) cells.
To evaluate the effect of viral miR-146a delivery on intraocular pressure (IOP) in vivo.
To assess the safety and efficacy of miR-146a as a potential glaucoma gene therapy.

Main Methods:

Human TM cells were treated with miR-146a mimics or inhibitors and subjected to cyclic mechanical stress (CMS).
Gene expression was analyzed using quantitative polymerase chain reaction (qPCR).
Replication-deficient adenoviral and lentiviral vectors expressing miR-146a were delivered to rat anterior chambers, with IOP monitored via rebound tonometry.

Main Results:

miR-146a modulated gene expression related to outflow homeostasis in TM cells, both at baseline and under CMS.
Viral delivery of miR-146a via both adenoviral and lentiviral vectors led to sustained IOP reduction (2.6–4.4 mmHg).
Long-term follow-up (>8 months) confirmed sustained IOP lowering (4.4 ± 2.9 mmHg) with no observed inflammation or visual acuity deficits.

Conclusions:

Intracameral delivery of miR-146a effectively lowers IOP in rats.
This approach demonstrates a long-term IOP-lowering effect without significant adverse events.
miR-146a gene therapy represents a promising strategy for future glaucoma treatment.