Modeling and optimization of radish root extract drying as peroxidase source using spouted bed dryer

Area of Science:

• Biotechnology
• Enzyme Technology
• Food Science

Background:

• Dried enzymes offer cost-effective storage and extended preservation for industrial applications.
• Garden radish root extract is a potential source of peroxidase enzyme.

Purpose of the Study:

• To optimize the spouted bed drying process for garden radish root extract to maximize residual enzyme activity (REA).
• To evaluate the impact of spouted bed drying on the catalytic activity, kinetic parameters, thermal stability, and shelf life of peroxidase enzyme.

Main Methods:

• Spouted bed dryer utilized for drying garden radish root extract.
• Response surface methodology (RSM) employed to optimize drying parameters (inlet air temperature and air flow rate ratio).
• Catalytic activity, optimum reaction conditions (pH, temperature), and kinetic parameters assessed for fresh and dried enzyme extracts.

Main Results:

• Maximum REA of 38.7% achieved at 50°C and an air flow rate ratio (Q) of 1.4.
• Optimum pH for dried enzyme extract decreased by 12.3%, while optimum temperature increased by 85.7% compared to fresh enzyme extract.
• Spouted bed drying significantly improved kinetic parameters, thermal stability, and shelf life of the peroxidase enzyme.

Conclusions:

• Spouted bed drying is a promising method for preserving heat-sensitive enzymes like peroxidase.
• The optimized drying process enhances the enzyme's stability and suitability for industrial applications.
• This technique offers a cost-effective approach to enzyme preservation.