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Alkaline extraction and reverse-phase high-performance liquid chromatography of adenine and pyridine nucleotides in
G Leoncini1, E Buzzi, M Maresca
1Institute of Biological Chemistry, University of Genoa, Italy.
Analytical Biochemistry
|September 1, 1987
Abstract:
The levels of adenine (ATP, ADP, AMP) and pyridine (NAD, NADH) nucleotides in human platelets have been measured by a simple and reproducible method. A rapid alkaline extraction allows a complete recovery of the compounds concerned. The metabolic ATP and ADP in the cytosolic fraction, the amount released upon thrombin stimulation, and the ADP bound to F-actin have also been evaluated. Analysis was performed by reverse-phase, isocratic high-performance liquid chromatography on a 5-microns Lichrosorb RP-18 column with uv detection at 254 nm.