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Mechanism of a Disassembly-Driven Sensing System Studied by Stopped-Flow Kinetics
Cara Gallo1, Suma S Thomas1, Allison J Selinger1
1Department of Chemistry and Centre for Advanced Materials and Related Technologies (CAMTEC), University of Victoria, P.O. Box 1700 STN CSC, Victoria, BC V8W 2Y2, Canada.
Abstract:
We carried out steady-state and stopped-flow photophysical measurements to determine the kinetics of a discrete disassembly driven turn-on fluorescent system. On and off rates for both DimerDye1 assembly and nicotine binding were determined. Relative rates for these competing processes provide insight on how this system can be optimized for sensing applications. Kinetics studies in artificial saliva showed that moving to more complex media has minimal effects on the sensing ability of the system.
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02:31Multi-Step Reactions
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