[Isolation and identification of an antileukemia substance from Coprinus radiatus]

Conditions for cultivation of basidiomycete Coprinus radiatus on agar media and its submersion fermentation were developed. An antibiotic was isolated from the fermentation broth filtrate by extraction with ethyl acetate followed by purification on a column with silica gel. The antibiotic had a pronounced in vivo effect on hemoblastosis La. Spectral analysis of the antibiotic (UV, IR, NMR and mass spectra) showed that it was identical to patulin (a toxin).